Scientific Program

Day 1 :

  • Medical Biotechnology and Biomedical Engineering|Plant Biotechnology |Agricultural and Environmental Biotechnology
Speaker
Biography:

Luis Lightbourn, president of the Instituto de Investigación Lightbourn located at Mexico, he is an expert in plant biotechnology, genomics, cell biology and has over 30 years of experience in plant biochemistry and molecular biology. Throughout his research career he has focused on how light regulates plant growth and development. In particular, he has made a major contribution to understanding the molecular responses of plants to ultraviolet radiation. Doctor Lightbourn has a range of expertise that has attracted invitations to contribute to a wide range of activities, including assessment of research strategy, industry consultation and government advice.

Abstract:

 Exposure to high-intensity UV-B irradiation induces the expression of many genes normally involved in defense, wounding, or general stress responses. However detailed processes of the linkage between light UV-B signaling and the upregulation of gene expression remain unclear. Therefore, the mechanism by which UV-B stress triggers the intracellular defense signaling pathway remains poorly understood. Moreover, according to our knowledge, no studies have analyzed the overall changes in global gene expression in bell pepper leaves exposed to UV-B. Molecular biological analyses have allowed us to draw a picture of UV stress responses in plants, and determination of the transcriptome has had a significant impact on this research field. Methodology & Theoretical Orientation: Therefore, deep sequencing, transcriptome assembly, and differential expression analysis were performed to investigate the regulatory mechanisms of Capsicum annuum in response to UV-B exposure. A global transcriptome analysis of the response to high-intensity UV-B irradiation was conducted and target genes regulated by UV-B were identified. Findings: We conducted a high-throughput screening analysis. After 1 h, 273 genes showed significantly different expression between control and treated plants, among these 111 were up-regulated and 162 were down-regulated; these were involved in several putative metabolic pathways related to biotic stress. After gene annotation and gene ontology enrichment analysis it was possible to determine that the UV-B radiation induced the expression of genes with functions in UV protection, including antioxidant enzymes, G proteins, primary and secondary metabolism, and transcription factors (Figure 1). Conclusion & Significance: Transcriptome profiling highlights possible signaling pathways and molecules for future research. These results opened ways of exploring the molecular mechanisms underlying the effects of UV-B irradiation on Capsicum and have great implications for further studies.

 

Mohsen Hajheidari

Max Planck Institute for Plant Breeding Research , Germany

Title: REDUCED COMPLEXITY, RCO: a leaf sculptor within the Brassicaceae family
Speaker
Biography:

Mohsen Hajheidari obtained his master’s degree in Plant Breeding at the University of Razi, Iran. Before undertaking his Ph.D in the group of Csaba Koncz in the Department of Plant Developmental Biology at the Max Planck Institute for Plant Breeding Research (MPIPZ), he was a scientific member at the Agricultural Biotechnology Research Institute of Iran. He completed his Ph.D. in Genetics in 2010 at the University of Cologne as an International Max Planck Research School (IMPRS) student. Following a postdoctoral study in the group of Csaba Koncz, he joined the group of Miltos Tsiantis in the Department of Comparative Development and Genetics in 2013. He is currently using comparative genetic approaches to uncover the genetic bases of leaf morphological complexity in plants. His goal is to combine evolutionary and computational approaches with comparative genetics and molecular physiology to further decipher plant-environment interaction.   

Abstract:

We are currently experiencing unprecedented climate change, which is a serious threat to our natural resources and food security at a time of rapid population increase. The sustainable food security requires a constant increase of genetic potential in crops. In order to increase the genetic potential of crops, exploring the genetic resources beyond major crops is a necessity. Leaf size and shape have an important effect on physiological processes such as photosynthesis and transpiration and thus on plant biomass. Therefore how morphological diversity of plant leaves is regulated constitutes an important branch of plant biology. In order to understand the genetic basis of morphological diversity in leaves, we have introduced a new model system C. hirsuta, which has dissected leaves with distinct leaflets, and it is a close relative of A. thaliana, which has simple leaves. Using comparative genetic approaches we discovered that a tandem duplication of the LATE MERISTEM IDENTITY 1 (LMI1) gene has given rise to two new copies in C. hirsuta. Diversification of the regulatory elements and coding sequence in one of the copies led to emergence of a novel transcription factor called Reduced Complexity, RCO. The RCO gene was lost in A. thaliana, contributing to leaf simplification in this species. In contrast to LMI1, which is expressed in the margins of leaflets, RCO is expressed at the base of leaflets and promotes leaflet formation through local growth regulation, at least in part by reprogramming the local phytohormone homeostasis. RCO expression is limited to leaves and its function is independent of shoot apical meristem development. Our data demonstrated that RCO is capable of improving photosynthetic efficiency, suggesting its contribution to adaptive evolution of leaf morphology. RCO studies could provide a basis for improvement of photosynthetic efficiency in crops

Mohammad Numan Ibne Asad

Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research-, Germany

Title: Characterization of anaerobic biotransformation of β-hexachlorocyclohexaneMohammad
Speaker
Biography:

Abstract:

Hexachlorocyclohexane (HCH) is a contaminant of concern worldwide. HCH has four main isomers α, β, δ, and γ-HCH. Since only γ-HCH (Lindane) has a specific pesticide activity, the purification of Lindane resulted in the production of other waste residues. β-HCH is the most persistent one, has relatively low water solubility and is considered highly carcinogenic and health hazardous. A large amount of β-HCH produced as a by-product which was dumped at landfill sites has caused heavy contamination in soil, groundwater, and atmosphere. In this study, we focused on the anaerobic degradation of β-HCH. Thus far, only one anaerobic, Dehalobacter sp. containing, a culture was reported in the literature. Contaminated soil was collected from a highly contaminated site in China and anaerobic microcosms were set up to enrich β-HCH degrading microorganisms. The degradation potential was evaluated by measuring the concentration of the products benzene and mono chlorobenzene (MCB). At the same time, cell growth was monitored by fluorescent microscopy. Illumina sequencing was done for the first and second generation and bacteria belonging to the Firmicutes, including Dehalobacter, Gelria, and Gracilibacter, were dominant. Additionally, the genomic DNA from an active, fourth generation, the β-HCH degrading culture was isolated and a 16s-rRNA clone library was prepared for subsequent sequencing to analyze the overall microbial diversity. Furthermore, compound-specific carbon stable isotope analysis (CSIA) will be applied to investigate the transformation pathway.

Speaker
Biography:

Abstract:

Cartilage is a dense connective tissue with limited selfrepair properties. Currently, the therapeutic use of autologous or allogenic chondrocytes makes up an alternative therapy to the pharmacological treatment. The design of a bioprinted 3D cartilage with chondrocytes and biodegradable biomaterials offers a new therapeutic alternative able of bridging the limitations of current therapies in the field. We have developed an enhanced printing processes-Injection Volume Filling (IVF) to increase the viability and survival of the cells when working with high temperature thermoplastics without the limitation of the scaffold geometry in contact with cells. We have demonstrated the viability of the printing process using chondrocytes for cartilage regeneration. An alginate-based hydrogel combined with human chondrocytes (isolated from osteoarthritis patients) was formulated as bioink-A and the polylactic acid as bioink-B. The bioprinting process was carried out with the REGEMAT V1 bioprinter (Regemat 3D, Granada-Spain) through a IVF. The printing capacity of the bioprinting plus the viability and cell proliferation of bioprinted chondrociytes was evaluated after five weeks by confocal microscopy and Alamar Blue Assay (Biorad). Results showed that the IVF process does not decrease the cell viability of the chondrocytes during the printing process as the cells do not have contact with the thermoplastic at elevated temperatures. The viability and cellular proliferation of the bioprinted artificial 3D cartilage increased after 5 weeks. In conclusion, this study demonstrates the potential use of Regemat V1 for 3D bioprinting of cartilage and the viability of bioprinted chondrocytes in the scaffolds for application in regenerative medicine.

Speaker
Biography:

International speaker on Biotechnology (mRNA) and its clinical applications with "No Option" patients. Served in various capacities in private and public practices throughout Mexico. MD degrees at Westhill University and the National Autonomous University of Mexico as qualified Physician Surgeon. Visiting Scholar in Dermatology, at the University of North Carolina, Chapel Hill. Advanced Fellow with the American Board of Anti-Ageing and Regenerative Medicine, Fellow in Stem Cell Medicine with both the American Academy of Anti-Ageing Medicine and University of South Florida. Master in Health Sciences at Aged Services Victoria University. Business Diplomate by the Einstein College of Australia.

Abstract:

As it has been previously demonstrated that coelectroporation of Xenopus laevis frog oocytes with normal cells and cancerous cell lines induces the expression of pluripotency markers and in experimental murine model studies that mRNA extract (Bioquantine®) purified from intra and extra-oocyte liquid phases of electroporated oocytes) showed potential as a treatment for a wide range of conditions, including Spinal Cord Injury (SCI) among others. The current study observed beneficial changes with Bioquantine® administration in a patient with a severe SCI. Pluripotent stem cells have therapeutic and regenerative potential in clinical situations CNS disorders even cancer. One method of reprogramming somatic cells into pluripotent stem cells is to expose them to extracts prepared from Xenopus laevis oocytes. The positive human findings for spinal cord injury with the results from previous animal studies with experimental models of traumatic brain injury and SCI respectively as our evidence and due to ethical reasons, legal restrictions and a limited number of patients, we were able to treat only a very small number of patients, deciding to include in our protocol the RestoreSensor SureScan to complete it. Based on the electrical stimulation for rehabilitation and regeneration after spinal cord injury published by Hamid and MacEwan, we designed an improved delivery method for the in-situ application of MSCs and Bioquantine® in combination with the RestoreSensor® SureScan®. To the present day the patient who suffered a complete section of spinal cord at T12-L1 shows an improvement in sensitivity, strength in striated muscle and smooth muscle connection, 13 months after the first treatment and 6 months after the placement of RestoreSensor® at the level of the lesion, showing an evident improvement on his therapy of physical rehabilitation (legs movement) on crawling forward and backwards and standing on his feet for the first time and showing a progressively important functionality on both limbs.

Olga Onyshchenko

Anti-age dentist, aesthetic injectionist, specialist in tissue regeneration BTI Biotechnology Institute , Ukraine

Title: Biological effects of plasma rich in growth factors in tissue regeneration. Benefits and easy use of biotechnologies in the daily practice of health practitioners.
Speaker
Biography:

Anti-age dentist, aesthetic injectionist, specialist in tissue regeneration .

Abstract:

Based on the expertise in anti-age dentistry and working with the biotechnology of the largest scientific background in regenerative medicine, which is plasma rich in growth factors, in the lecture will be presented the range of properties of this biological therapy for individualized medicine. It was proven to be effective in regeneration of tissues differentiated from the mesenchymal stem cells (cells composing muscular-skeletal system, like osteoblasts, adipocytes and chondrocytes as well as myocytes and neurons), the capacity of which ones to proliferate and differentiate is known to decrease with the age of the patient. The lecture will present the data of scientific studies of different levels to prove the regenerative potential of patient’s own blood proteins (growth factors) to perform the safe and high-quality treatment in different areas of medicine while reducing the possibilities of side effects and performing less- or non-invasive procedures to our patients. Additionally will be presented the set of clinical cases illustrating the theoretical part of the lecture and how easy it is to introduce such biotechnologies into the daily practice of the professionals in dental, surgical, dermatological, traumatological, ophthalmological spheres, as well as sports medicine and focusing on own clinical results in esthetic and anti-age medicine.

  • Food Biotechnology and Nutritional Science | Laboratory Methods for Biotechnology|Stem Cell Biotechnology
Speaker
Biography:

Biologist Ricardo Rangel has 20 years of experience in the development of biotechnological platforms for the isolation of adult stem cells, as well as in  the development of preclinical and clinical protocols for private industry. He is CEO of the company Stemcylab and CryoVida Mexico.

 

Abstract:

The biotechnological advance around the knowledge of the mesenchymal stem cells has led us to explore the bio-activity of diverse molecules and subcellular derivatives thereof. Currently, more than 800 clinical trials are being carried out according to the clinicaltrials.gov database, which demonstrates the enormous interest that exists in regenerative medicine. In the last ten years a large amount of evidence has accumulated of the therapeutic effect that can be attributed to mesenchymal stem cells. These cells can be isolated from various tissue sources, the most common being those obtained from the bone marrow, adipose tissue, dental pulp and embryonic attachments such as placenta and Wharton's gelatin.In the endometrial tissue, it has been proven since 2008, that it is a source of mesenchymal stem cells with a high therapeutic value since it possesses properties superior to those of other sources of these cells. One of them is the large number of molecules related to angiogenic processes, which are altered in conditions such as ulcers secondary to type II diabetes mellitus. In addition to producing anti-inflammatory and immunomodulation molecules, important in the regenerative process. Regenerative cell-free medicine in an emerging discipline that raises the use of stem cell derivatives as therapeutic agents. Microvesicles are subcellular components that have a great importance in inter-cellular communication at the local and systemic level. These microvesicles are loaded with bioactive molecules and microRNAs, which have been shown to be sufficient to allow the processes of immunomodulation and cell regeneration. For the first time, here we demonstrate the important regenerative potenical of microvesicles isolated from the mesenchymal stem cells of endometrial tissue in the resolution of deep wounds in diabetic patients. (Case reports).

Speaker
Biography:

Dr. Akuma Saningong is a master communicator and expert in unlocking and maximizing your potential by Bridging Science and Personal Development. He findtunes the knowledge from Quantum Physics and the Biology of Epigenetics for you to live Your Greatness and Full Potential. 

Abstract:

The science of epigenetics, which literally means „control above genetics“, profoundly changes our understanding of how life is controled. Environmental influences including nutrition, stress, and emotions, can modify genes without changing their basic blueprint. The environment serves as a „contractor“ who reads and engages those genetic blueprints and is ultimately responsible for the character of a cells’s life. It is a single cell’s „awareness“ of the environment that primaruly sets into motion the mechanisms of life.In fact epigenetics, the study of the molecular mechanisms by which the environment controls gene activity, is today one of the most active areas of scientific reserach. Epigentics reveals that when we change our perceptions and environment, our genes can change as well.  The idea that our perceptions override our genes is now at the forefront of medical research. Everything we've left out of the medical model - energy, thoughts, spirit - now turns out to be the primary mechanism of interaction with physical reality.In my keynote, I share with the basic concepts of epigenetics and how it is changing our understanding to health and disease. And above all, having same genes doesn’t mean same having the same fate.

  • Microbial and Biochemical Technology

Session Introduction

Debora Colombi

Scientific Director at GENOTYPING BIOTECNOLOGIA , Brazil

Title: Nanoparticles based bacterial identification kit
Speaker
Biography:

Débora Colombi is a Brazilian entrepreneur who founded two companies: Genotyping and BPI. She has degree in Biomedicine from UNIFESP and a Masters’ and PhD degree in Biochemistry and Molecular Biology from USP and a post doctorate in Genetics from UNESP. The objective of her actual project is the development of a kit for the detection of bacterial contaminants present in the fermentation tanks of sugarcane industries. The company is already expanding the kit to other markets to facilitate the identification of contaminants in loco. Her companies offer genomic solutions for researchers and other companies, in addition to human genetic diagnoses. 

Abstract:

Brazil is the second largest ethanol producer in the world, using yeast fermentation by sugar cane, Brazil produces 26 billion liters per year, which means a market of 20 billion dollars. Bacterial contamination is a relevant factor in the industrial process, as this can cause damage to the transformation of raw material fermentation in other undesirable substances or consuming part of ethanol, which leads to losses in fermentation yield, causing financial loss. Methods used today to measure bacterial contamination are microscopic counting, plating techniques and MC kit. The problems of these methods are very high response time (one to five days), and the lack of bacteria identification due to measure of sub-products. Our solution aims at the production of a kit for rapid monitoring and identification of contaminant microorganisms based on immunosensor colorimetric change. Biosensors based on gold nanoparticles can be bio conjugated with various ligands such as nucleic acids and antibodies. After the bio conjugation, they start forming aggregates, which shifts the absorption band to ca. 600–800 nm. This change can be observed by the naked eye or measured quantitatively with an ultraviolet-visible spectrophotometer Measure will be carried out in half an hour, in this way alcohol industry will be able to have more timely interventions to stop contamination and use less antibiotics in controlling contaminants. Our experiment indicates that bacteria can be detected quickly and accurately without any amplification or enrichment in around 100 cfu/mL level with excellent discrimination against any other bacteria. In this work we have demonstrated a universal method for detection bacteria using gold nanoparticles. This proves to be a quick, simple and clean way to detect bacteria in real time.